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PRMT5 gene expression positively correlates to DDR gene expression in cancer cells. A, Heat map of Pearson correlation coefficients between PRMT5 and HRD signature genes for breast, ovarian, pancreatic, and prostate cancer cell lines from CCLE (heat map is sum ranked). B, Dot plots showing PRMT5 expression relative to key DDR genes in ovarian and breast cancer cell lines (dots represent cell lines). C, Pearson correlation coefficient r values were deconvoluted and shown in bar graph. D, mRNA expression of select correlated DDR genes by qPCR following transient genetic knockdown of PRMT5 by shRNA in MCF7 cells ( n = 2).

Journal: Cancer Research Communications

Article Title: PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies

doi: 10.1158/2767-9764.CRC-23-0070

Figure Lengend Snippet: PRMT5 gene expression positively correlates to DDR gene expression in cancer cells. A, Heat map of Pearson correlation coefficients between PRMT5 and HRD signature genes for breast, ovarian, pancreatic, and prostate cancer cell lines from CCLE (heat map is sum ranked). B, Dot plots showing PRMT5 expression relative to key DDR genes in ovarian and breast cancer cell lines (dots represent cell lines). C, Pearson correlation coefficient r values were deconvoluted and shown in bar graph. D, mRNA expression of select correlated DDR genes by qPCR following transient genetic knockdown of PRMT5 by shRNA in MCF7 cells ( n = 2).

Article Snippet: Lentiviral short hairpin RNA (shRNA) particles (Sigma-Aldrich) for PRMT5 and empty vector shRNA controls were purchased from Sigma.

Techniques: Expressing, shRNA